Figure 1
Plasma IgG responses to gp120, V1V2, and V3 in vaccine recipients in the course of 7 immunization doses. Plasma samples from 23 VAX003 (AIDSVAX® B/E) and 23 VAX004 (AIDSVAX® B/B) vaccine recipients were tested at 1:100 dilution for ELISA IgG reactivity against gp120 protein (B.JRFL), V1V2-gp70 protein (B.Case A2), and cyclic full-length 35-mer V3 peptide (B.MN). Samples were tested in duplicate against each antigen in 1 or 2 independent experiments; averages from all experiments are shown. (a) Plasma samples collected before immunization (A02) and 2 weeks after each of 7 vaccine doses (A03, A05, A07, A09, A11, A13, A15) were tested. (b) Longitudinal analysis assessed gp120-, V1V2-, and V3-binding Ab strengths in VAX003 vaccinees. (c) Longitudinal analysis of gp120-, V1V2-, and V3- binding Ab strengths was also performed for VAX004 vaccinees. Comparison of Ab strengths between A15 (final) and A07 or A09 (peak) was done by t test (paired, 2-tailed). Average Ab levels are highlighted by bold black lines. Percentages of responders (% positive) are shown below each graph. Cutoff values (dotted lines) were based on mean and 3 standard deviations of placebo recipients. OD405, optical density at 405 nm. ****P < 0.0001; NS: not significant (P ≥ 0.05).
