Figure 2 | Scientific Reports

Figure 2

From: Dengue virus-induced ER stress is required for autophagy activation, viral replication, and pathogenesis both in vitro and in vivo

Figure 2

Modulation of UPR pathways during DENV2 infection is a general event in various human cell lines. (A) Hepatoma Huh7 cells and (B) lung cancer A549 cells were infected with DENV2 (MOI = 10) and the ER stress-related markers including GRP78, ATF4, IRE1α and ATF6α as well as the autophagy marker LC3-II/LC3-I and DENV2 NS1 protein expression was evaluated by Western blotting using the specific antibodies. During ATF6α activation, it is cleaved from the total form (90 kDa) to the active form (50 kDa). The anti-ATF6α antibody used in this study can detect the total form ATF6α only. The number under the band is the quantification of band intensity after normalization with β-actin.

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