Figure 7
DENV2 infection induces both ER stress and autophagy in ICR suckling mice. Six-day-old ICR suckling mice (Mock n = 6, DENV2 n = 6) were i.c. inoculated with DENV2 (2.5 × 105 PFU/mice). The (A) body weight, and (B) clinical score were monitored every day after inoculation until day 5. Disease symptoms were scored as following: 0 for healthy. 1 for lightly sick (losing weight and ruffled hair). 2 for moving slowly and reduced mobility. 3 for moving difficulty, forelimb or hindlimb weakness, 4 for paralysis and mortally ill. 5 for death. Mock group was given equal volume of 2% FBS/DMEM. (C) The mice were sacrificed at day 5 p.i. The expression of GRP78, LC3-II/LC3-I and NS1 was determined by Western blotting. The number under the band is the quantification of band intensity after normalization with β-actin. M: without DENV2 infection; V: with DENV2 infection.
