Figure 3
From: Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
Evidence of ferroptosis in PC12 cells after co-treatment with t-BHP. PC12 cells were treated with t-BHP (100 μM) for 1 h with or without Fer-1 (1 μM)/DFO (100 μM)/FAC (1 μg·ml−1) pretreatment for 24 h. Generation of lipid ROS (A and B) was determined by the C11-BODIPY (581/591) probe. The ratio of GSH to GSSG (I) was determined by a luminometer kit (C). The expression of Gpx4 (D–F) was detected by western blot analysis (the displayed blots are cropped, and the full-length blots are included in the Supplementary Information file). **P < 0.01; *P < 0.05; Fer-1, ferrostatin-1; DFO, deferoxamine; FAC, ferric ammonium citrate.
