Figure 4
From: Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
Evidence of mitochondrial dysfucntion in PC12 cells after co-treatment with t-BHP. Mitochondrial membrane potential and mitochondrial ROS (A and B) were detected by JC-1 and MitoSOX probes. ATP generation (E) was detected by a luminometer kit. The expression of mitochondria-related proteins (C and D) was determined by western blot analysis (the displayed blots are cropped, and the full-length blots are included in the Supplementary Information file). The activity of caspase-3/-7 was detected by a luminometer kit (F). **P < 0.01; n.s, no significance.
