Figure 5
From: Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
Evidence against t-BHP-induced autophagy and necroptosis in PC12 cells. PC12 cells were treated with t-BHP (100 μM) for 1 h with or without CQ (30 μM) pretreatment for 24 h. The autophagic vacuoles (A) and the expression of LC-3I and LC-3II (B) were determined by MDC staining and western blot analysis (the displayed blots are cropped, and the full-length blots are included in the Supplementary Information file). The expression of necroptosis-related proteins (C) was determined by western blot analysis (the displayed blots are cropped, and the full-length blots are included in the Supplementary Information file). n.s, no significance.
