Figure 7
From: Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
JNK1/2 and ERK1/2 inhibitors inhibited t-BHP-induced ferroptosis and mitochondrial dysfunction. PC12 cells were treated with t-BHP (100 μM) for 1 h with or without SP600125 (10 μM)/U0126 (10 μM) pretreatment for 24 h. Mitochondrial membrane potential and mitochondrial ROS (A–C) were detected by JC-1 and MitoSOX probes. ATP generation (C) was detected by a luminometer kit. The generation of lipid ROS (E and F), the ratio of GSH to GSSG (D), and the expression of Gpx4 (B) were determined by the C11-BODIPY (581/591) probe, luminometer kit and western blot analysis (the displayed blots are cropped, and the full-length blots are included in the Supplementary Information file), respectively. **P < 0.01; *P < 0.05.
