Figure 8
From: Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
t-BHP-induced cell death routine in PC12 cells. In PC12 cells treated with 100 μM t-BHP for 1 h, the membrane of PC12 cells underwent lipid peroxidation, accompanied by GSH depletion, reduced Gpx4 expression, and increased lipid ROS. These lethal ROS further damaged the mitochondria, increased mitochondrial ROS generation, decreased the mitochondrial membrane potential, and increased the release of cytochrome c to the cytoplasm, ultimately leading to cell death. Moreover, in this death process, SP600125 and U0126 acted upstream of these events, and Fer-1 acted upstream of mitochondrial dysfunction.
