Figure 4
From: Inactivated rotavirus vaccine by parenteral administration induces mucosal immunity in mice
IRV vaccination leads to elevated levels of rotavirus-specific IgG and IgG subclass in sera of mice. Peripheral blood was collected before the mice received the first vaccination dose (day 0), before the second and third vaccination dose (day 21, day 42) and at the study endpoint (day 63). (a) Individual serum samples or (b) pooled serum samples from 5 mice were tested for IgG and IgG1 and IgG2a, respectively, by ELISA as described in the text. (c) IgG2a:IgG1 ratio was calculated from antibody levels detected in (b). Shown in (a) are mean and s.d. of n = 5. Mann-Whitney test was used for statistical analysis. MN: microneedle; IM: intramuscular; < : below detection limit; ns: not significant; *p ≤ 0.05; **p ≤ 0.01.
