Figure 1 | Scientific Reports

Figure 1

From: 19F-perfluorocarbon-labeled human peripheral blood mononuclear cells can be detected in vivo using clinical MRI parameters in a therapeutic cell setting

Figure 1

All cell lineages within human PBMC important for antigen presentation label with 19F-PFC. Human PBMC were cultured overnight with a red fluorescent version of the 19F-PFC and flow cytometry was used to qualitatively assess 19F-PFC incorporation. Three different cell concentrations (2–10 × 106 cells/mL) and three different 19F-PFC labeling concentrations were employed (2.5–7.5 mg/mL). (a,b) CD3+ and CD19+ T and B cell lymphocytes (a) and CD11c+ dendritic cells (b) label with a high percentage regardless of cell or 19F-PFC concentration. (c) CD14+ monocytes label most consistently at a high percentage (>98%) when a cell concentration of 5 × 106 cells/mL and a 19F-PFC concentration of 5 mg/mL is used (n = 3, mean ± SEM).

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