Figure 6
Cellular distribution of eNOS and NADPH diaphorase activity in WT vs. CGL4 fibroblasts. (a,b) Immunogold detection of Cav1 (a; 10 nm gold grains, arrows) in plasma membrane sheets obtained by rip/flip-technique. Note that in CGL4-fibroblasts, membrane is virtually devoid of a Cav1 signal (B); scale bars 100 nm. (c,d) Cellular distribution of transfected eNOS with a membrane-bound signal in WT (c, arrows) as opposed to the more perinuclear signal in CGL4-fibroblasts (d, arrows); scale bars 10 µm. (e,f) Enhanced NADPH-diaphorase activity in CGL4-fibroblasts (f) suggests enhanced NOS activity in Cav1 deficiency; representative results of three independent experiments.
