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Figure 1

From: Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients

Figure 1

MALDI-TOF mass spectra of a patient’s serum (upper) and peritoneal effluent (lower) protein-derived N-glycans. After enzymatic release of glycans, sialic acids were stabilised in linkage-specific manner and analysed by positive-ion reflectron mode MALDI-TOF-MS. Blue square, N-acetylglucosamine; yellow circle, galactose; green circle, mannose; red triangle, fucose; purple diamond oriented to the right, α2,6-linked N-acetylneuraminic acid; purple diamond oriented to the left, α2,3-linked N-acetylneuraminic acid; asterisks indicate peaks of non-N-glycan origin. Structures are proposed for the 12 most abundant species in the upper mass spectrum, while the lower panel contains 11 additional structures in the two zoomed areas, i.e. peaks 1, 4, 6–8, 10, 12, 15–17, and 21. For the complete list of, in total, 26 detected glycan species, see Supporting Information Table S-1.

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