Table 4 Antisera used for western blots.
Antiserum | Host | Source/reference | Cat # | Dilution |
|---|---|---|---|---|
Primary antisera: | ||||
VGLUT1 | Rabbit | n.a. | 1:10,000 | |
VGLUT2 | Rabbit | n.a. | 1:800 | |
VIAAT | Rabbit | Gift from B.Gasnier86 | n.a. | 1:10,000 |
α-tubulin | Mouse | Sigma Aldrich | T5168 | 1:500,000 |
EAAT2 | Mouse | Santa Cruz Biotechnology | sc-365634 | 1:20,000 |
PSD95 | Mouse | Millipore | MAB1596 | 1:10,000 |
Synaptophysin | Mouse | Synaptic System | 101 111 | 1:40,000 |
Secondary antisera: | ||||
Horseradish peroxidase | Mouse | Sigma Aldrich | A9044 | 1:20,000 |
Horseradish peroxidase | Rabbit | Sigma Aldrich | A6153 | 1:20,000 |
IRDye 700DX | Mouse | Rockland | 610–130–124 | 1:5,000 |
IRDye 800 | Rabbit | Rockland | 611–131–122 | 1:5,000 |
