Figure 7 | Scientific Reports

Figure 7

From: Investigation of 20S-hydroxyvitamin D3 analogs and their 1α-OH derivatives as potent vitamin D receptor agonists with anti-inflammatory activities

Figure 7

20S(OH)D3 analogs upregulate the expression of CYP24A1 encoding the vitamin D catabolic enzyme, CYP24A1, with varying efficiencies. HaCaT cells were treated with 100 nM concentrations of analogs, 20S(OH)D3, 1,25(OH)2D3 or 22-Oxa, or with DMSO only (vehicle) as a control. The mRNA was isolated and the RT-PCR was performed using specific primers for the CYP24A1 gene (see Methods). Data are presented as mean ± SE (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the control.

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