Figure 2
From: Structural basis of thalidomide enantiomer binding to cereblon
Effects of deuterium-substituted (S)- and (R)-thalidomides on IKZF3 degradation and CRBN-IKZF3 binding. (a) Effects of deuterated (S)- and (R)-thalidomide enantiomers, (S)-D-Thal and (R)-D-Thal, on IKZF3 protein levels in MM1S cells after 12-h treatment. IB, immunoblotting. (b) Co-immunoprecipitation of FLAG-HA-CRBN in the presence of each deuterated thalidomide enantiomer, (S)-D-Thal or (S)-D-Thal. IP, immunoprecipitation. Full-length blots in (a) and (b) are presented in Supplementary Fig. 9.
