Figure 3

(S)- and (R)-thalidomides bound to CRBN TBD. (a) Stereo views of (S)-thalidomide (yellow) and (R)-thalidomide (green) molecules bound to mouse CRBN TBD in the crystal with composite omit maps (2mFo-DFc) shown in 1σ contour (blue). (b) Stereo view of mouse CRBN TBD (cyan) bound to (S)-thalidomide (yellow). Side chains of residues at the tri-Trp pocket are shown as stick models. (c) Close-up view of a (S)-thalidomide molecule (yellow) bound to the tri-Trp pocket (cyan). Residues forming the tri-Trp pocket are shown as stick models (cyan for C, blue for N, and red for O). The (S)-thalidomide molecule is shown as a stick model (yellow for C, blue for N, and red for O). Two hydrogen bonds formed between the protein and the glutarimide moiety of thalidomide are represented as red dotted lines. (d) As in c but for (R)-thalidomide (green for C, blue for N, and red for O) bound to the tri-Trp pocket (magenta).