Figure 7
From: Structural basis of thalidomide enantiomer binding to cereblon
(S)-thalidomide treatment causes more sever developmental defects in zebrafish than (R)-thalidomide. (a) Dorsal views of pectoral fins of 72-hpf embryos. Fins are indicated by arrowheads. The teratogenic effects resulting from treatment with thalidomide are classified into three categories. Fins that stretched out from the body wall and were more than 85% in length compared to control fins were defined as “no effect.” Fins that stretched out but were shortened to 60–85% of control length were defined as “mild” phenotypes. Fins that showed disc-like morphology and were shortened to less than 60% of control length were defined as “severe” phenotypes. (b) Incidence of pectoral fin malformations. Treatments with 200 μM or 400 μM (S)-thalidomide (S200 and S400, respectively) induce more severe effects than treatments with 200 μM or 400 μM (R)-thalidomide (R200 and R400, respectively) in 72-hpf embryos.
