Table 1 Geltrex™, cRGDfK-PAPA, StemAdhere™ and Synthemax™ are ranked as hPSC culture surfaces from most (1) to least (4) preferred with regards to cost, preparation, shelf life and the requirement for scraping to harvest cells. For example, Geltrex is the cheapest and is therefore ranked 1 (most preferred) for cost, while StemAdhereTM requires the least scraping and is therefore ranked 1 for scraping. Cost was ranked according to a recently published comparison57, preparation was ranked according to the number of steps involved in the coating procedures at the point of passage (commercially available cRGDfK-PAPA would be prepared as a complete coating onto which cells would be seeded directly), the reported shelf lives of commercially available surfaces were compared to the tested shelf life of cRGDfK-PAPA (data not shown) while the cell scraping rankings are a summary of the results presented in Fig. 6B.
From: Long-Term Maintenance of Human Pluripotent Stem Cells on cRGDfK-Presenting Synthetic Surfaces
Surface | Cost | Preparation | Shelf life | Cell scraping |
---|---|---|---|---|
Geltrex™ | 1 | 4 | 3 | 2 |
cRGDfK-PAPA | Unknown (likely less than Synthemax™) | 1 | 1 | 2 |
StemAdhere™ | 2 | 3 | 4 | 1 |
Synthemax™ | 3 | 2 | 2 | 4 |