Figure 2

Ca²⁺ influx in adrenal gland chromaffin cells from EPHB6 KO and WT mice. Adrenal gland chromaffin cells were loaded with Fura2-AM, and stimulated with acetylcholine (5 mmol/L) at 37 °C. The cells were imaged for 120 seconds at a rate of one measurement per two seconds. Arrows indicate the time points where acetylcholine was added. Signals from more than 15 randomly selected cells per group were recorded, and the results expressed as means ± S.E. of ratios of fluorescence intensity at 510 nm excited by 340 nm versus 380 nm. The data were analyzed in a linear mixed-effect model, with genotype, individual cells, sex and time as qualitative factors. P-values are indicated. The experiments were repeated at least three times. Data from representative experiments are shown. (A) Male but not female KO adrenal gland chromaffin cells present reduced Ca²⁺ influx compared to their WT counterparts. (B) Castration reverses low Ca²⁺ influx in KO adrenal gland chromaffin cells to normal but has no effect on WT adrenal gland chromaffin cells. The S.E. of the solid line curve representing male WT adrenal gland chromaffin cell Ca²⁺-influx in the left panel is omitted for better visualization.