Figure 3

Effects of sex hormones on Ca²⁺ flux in adrenal gland chromaffin cells from EPHB6 KO and WT mice. The experiment procedures and data presentation are the same as described in Fig. 2. The data were analyzed in a linear mixed-effect model, with genotype, individual cells, sex, sex hormone and time as qualitative factors. (A) Fifteen-minute testosterone treatment rapidly lowers Ca²⁺ influx in castrated KO but not in WT adrenal gland chromaffin cells. (B) Cell membrane-impermeable, BSA-conjugated testosterone rapidly (within 15 minutes) lowers Ca²⁺ influx in castrated KO but not WT adrenal gland chromaffin cells. (C) Fifteen-minute testosterone treatment rapidly lowers Ca²⁺ influx in female KO but not WT adrenal gland chromaffin cells. (D) Cell membrane-impermeable, BSA-conjugated testosterone rapidly (within 15 minutes) lowers Ca²⁺ influx in female KO but not WT adrenal gland chromaffin cells. (E) Short-term (15-minute) estrogen treatment does not affect male KO and WT adrenal gland chromaffin cell Ca²⁺ influx. F. Long-term (24-hour) estrogen treatment does not affect male KO and WT adrenal gland chromaffin cell Ca²⁺ influx.