Figure 4

Voltage-dependent calcium current densities in adrenal gland chromaffin cells from WT and EPHB6 KO mice, with or without castration. Total calcium current densities recorded in adrenal gland chromaffin cells with the perforated patch technique. Current-voltage curves are presented as means ± S.E. of I(pA/pF). Curves are generated from pooled data from 8–10 cells from 2–3 mice: WT (n = 11 cells from 3 mice), KO (n = 8 cells from 2 mice), castrated WT (n = 10 cells from 3 mice), castrated KO (n = 8 cells from 3 mice). *p < 0.05 (2-tailed Student’s t test). (A) Male KO adrenal gland chromaffin cells showed higher Ca²⁺current densities compared to their WT counterparts. (B) Castration significantly increased voltage-gated Ca²⁺ current densities in WT adrenal gland chromaffin cells. (C) Castration did not significantly increase voltage-gated Ca²⁺ current densities in KO adrenal gland chromaffin cells. (D) Voltage-gated Ca²⁺ current densities became similar in adrenal gland chromaffin cells from castrated WT and KO adrenal gland chromaffin cells.