Figure 5 | Scientific Reports

Figure 5

From: Supra-pharmacological concentration of capsaicin stimulates brown adipogenesis through induction of endoplasmic reticulum stress

Figure 5

Stimulation of brown adipogenesis by supra-pharmacological capsaicin in a Trpv1-independent manner. (A) HB2 brown preadipocytes loaded with Fluo-8 AM were treated with capsaicin (100 μM) or A23187 (1 μM) in the absence of extracellular calcium and in the presence or absence of I-RTX (1 μM), and cytosolic calcium level was evaluated. (B–D) HB2 brown preadipocytes were cultured with 100 μM of capsaicin in the presence or absence of I-RTX (1 μM) during brown adipogenesis. (B) Oil Red O staining of cells on day 8 was performed and the dye intensity was quantified (n = 2). (C and D) Expression levels of Ucp1 (C) or Pref-1 (D) on day 8 were examined by RT-qPCR analysis. Black bar: vehicle; Hatched bar: capsaicin. The data are presented as the mean ± SE (n = 4). **P < 0.01 vs. cells treated with vehicle and corresponding reagent (vehicle or I-RTX). ‡P < 0.01 vs. cells treated with vehicle and corresponding reagent (vehicle or capsaicin).

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