Figure 6 | Scientific Reports

Figure 6

From: Supra-pharmacological concentration of capsaicin stimulates brown adipogenesis through induction of endoplasmic reticulum stress

Figure 6

Induction of ER stress by supra-pharmacological capsaicin. (A) HB2 brown preadipocytes were treated with the indicated reagent, i.e., capsaicin (100 μM), A23187 (500 nM), dithiothreitol (150 mM), tunicamycin (2 μg/mL) or thapsigargin (100 nM), for 4 h. Xbp1 expression was evaluated by RT-PCR analysis. Polyacrylamide gel electrophoresis and subsequent ethidium bromide staining were performed to distinguish sXbp1 from uXbp1. The cropped image of RT-PCR analysis is shown because of space limitations; image of the full-length gel is Supplementary Fig. S7. (B–D) HB2 brown preadipocytes were treated with the indicated concentration of capsaicin for 4 h. Expression levels of sXbp1 (B), uXbp1 (C) or Chop (D) were examined by RT-qPCR analysis. The data are presented as the mean ± SE (n = 4). *P < 0.05 vs. control cells.

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