Figure 8

Stimulation of Creb phosphorylation by supra-pharmacological capsaicin. HB2 brown preadipocytes on day 0 were cultured with or without 100 μM capsaicin in the presence of insulin for the indicated time (A) or with the indicated concentration of capsaicin in the presence of insulin for 30 min (B). Expression level of phosphorylated (p) and total Erk, p38 and Jnk was examined by Western blot analysis. In addition, expression of pCreb as well as β-actin as the loading control was also evaluated. Representative results are shown. The cropped images of Western blot analysis are shown because of space limitations; images of the full-length blot are Supplementary Fig. S8 (A) and Fig. S9 (B).