Figure 3

The use of CXCR4^-CORIN⁺CD166⁺ markers for enrichment of mDA NPCs. (A) Identification of mDA NPC surface markers by single antibody sorting. Day 14 BJ-RiPS cells were stained with antibodies corresponding to the putative surface marker genes shown in Fig. 2B. After staining, cells were FACS purified into positive and negative populations, and stained with FOXA2. n = 3, *(p < 0.05). White bar = 50 um. (B) CXCR4^-CORIN⁺CD166⁺ FACS purification scheme. (C) FOXA2⁺% quantification comparison between unsorted, CORIN⁺, and triple (CXCR4^-CORIN⁺CD166⁺) cells from 1016A, 15b, 18a, and BJ-RiPS-derived NPCs. N.S. (Non-significant), *(p < 0.05) and **(p < 0.005) using t-test (n = 3). (D) Representative photos of BJ-RiPS-derived NPCs from Fig. 3C. White bar = 50 um. (E) Representative photos from in vitro differentiation following the FACS purification. The BJ-RiPS cells were fixed at day 42 and stained with antibodies against MAP2, TH, and DAPI. White bar = 50 um. (F) Quantification of Fig. 3E. Using t-test, n = 3, *(p < 0.05). Error bars represent SEM.