Figure 2

(a) Western blot was performed with AV2 antisera to detect the expression of AV2 in N. benthamiana leaves samples infiltrated with PVX-AV2, PVX-AV2Δ10N at 14 dpi. PVX, mock infiltrated samples; and purified AV2 protein were used as negative control and positive controls, respectively. (b) DAB and NBT staining for detection of H2O2 and O2− radicals in PVX-AV2, PVX-AV2Δ10N and PVX inoculated N. benthamiana at 14 dpi, showing the higher accumulation of ROS in PVX-AV2 infiltrated leaves.