Figure 2
From: Chikungunya virus nsP1 interacts directly with nsP2 and modulates its ATPase activity
CHIKV nsP2-NT directly interacts with nsP1-WT in vitro. (a) Coomassie stained 12% SDS-PAGE showing recombinant nsP1-WT, thrombin digested nsP1-WT, nsP2-NT and nsP2-CT. Purified recombinant nsP1-WT and nsP2-NT were incubated for interaction in vitro and were either immunoprecipitated with anti-nsP2 mAb (b) or anti-nsP1 pAb (c) and the same protein or the respective interacting partners were detected by the Western blot. The CHIKV nsP2-CT was incubated with nsP1-WT in vitro, the protein complexes were immunoprecipitated with either nsP2-CT pAb (d) or nsP1 pAb (e) and detection was carried out with specific antibodies in the Western blot. (f) Thrombine digested and purified nsP1-WT and nsP2-NT were subjected to IP using anti-nsP2 mAb and Western blot was carried out using anti-nsP1 pAb and anti-nsP2 mAb.
