Figure 3
From: Chikungunya virus nsP1 interacts directly with nsP2 and modulates its ATPase activity
The residues between 170 to 288 aa of nsP1 is responsible for the interaction with nsP2-NT. (a) Graphical representation of different truncations of CHIKV nsP1 indicating specific amino acid positions. (b) The CHIKV nsP1 truncations were over-expressed in BL-21 cells. The Western blot showing the different CHIKV nsP1 truncated proteins using anti-His mAb. (c) Both the CHIKV nsP1 truncated and nsP2-NT proteins were incubated at 4 °C for 2 hr for interaction in vitro. The protein complexes were immunoprecipitated using anti-nsP2 mAb, separated in 12% SDS-PAGE and the Western blot was probed with anti-His mAb. The lower panel shows the negative control where different nsP1 trancated proteins were immunoprecipitated with nsP2 mAb and beads. (d) Coomassie stained 12% SDS-PAGE showing the purified fragment of nsP1 (170-288). (e) The 170-288 aa long purified fragment of nsP1 was incubated with purified nsP2-NT. The protein complex was immunoprecipitated with anti-nsP2 mAb and the Western blot was probed with anti-His mAb. The bead (with nsP2 mAb) was considered as negative control.
