Figure 3
CSs reduce expression of essential HIV-1 structural and regulatory proteins. HeLa rtTA-HIV-ΔMls cells were treated with ~IC80s of CSs (per Methods), 100 nM of RIDK-21 or -27, or DMSO for 4 h prior to Dox induction for 20 h as indicated. Cell extracts were analyzed by immunoblot of (a) HIV-1 structural proteins: Env (gp160/gp120) and Gag (p41/p24), and (b–d) viral regulatory factors: Rev (p19, b) and Tat (p16/p14, c,d). (d) Graph quantitating Tat expression. Results from (a–d) are from n ≥ 3, mean, s.e.m. GAPDH/tubulin served as internal loading control and for normalization of these data. (a and d) Lanes were cropped and assembled from the same blot per box from Supplementary Figure S4a,b and c, respectively.
