Figure 1

(A) Immunofluorescent (left) and flow cytometry (right) detection of the EpCAM expression in MCF7 cells and the FRα expression in A2780 cells. EpCAM and FRα stained with Alexa Fluor^® 488 are green at an excitation of 488 nm, and the nuclei stained with DAPI are blue at an excitation of 405 nm. As a negative control, we used EpCAM to stain Jurkat cells, which are EpCAM negatively expressed, and we used FRα to stain A549 cells, which are FRα negatively expressed. Histograms of flow cytometric analysis: MCF7 cells (top) were stained with anti-EpCAM antibodies (red) and A2780 cells (bottom) were stained with anti-FRα antibodies (red), the negative control was autofluorescent (orange), and the isotype was mouse IgG plus secondary antibody (blue). (B) Schematic of our CTCs enrichment strategy. Anti-EpCAM-MNs and Anti-FRα-MNs was added to the whole blood and after incubation, magnetic separation and fluorescence identification, the cells of DAPI⁺/CK⁺/CD45⁻ were defined as CTC.