Figure 5
The effects of TFP on the apoptosis of SWOZ2, SWOZ2-BCNU and U251 cells. (A) Flow cytometry analysis of Annexin V-positive cells in SWOZ2, SWOZ2-BCNU and U251 cells incubated with TFP or control. (B–D) The rate of Annexin V-positive cells in SWOZ2, SWOZ2-BCNU and U251 cells after treatment. All Annexin V-positive cells were considered apoptotic cells, and their percentage was calculated among the total number of cells. (E) TUNEL staining assay on SWOZ2, SWOZ2-BCNU and U251 cells after TFP treatment (400×). (F–H) The rate of apoptotic cells in SWOZ2, SWOZ2-BCNU and U251 cells after treatment by TUNEL assay. Each bar represents the mean ± SD of three experiments. *P < 0.05 compared with the control.
