Figure 7
TFP treatment downregulated the intracellular Ca2+ concentration in glioma cells. (A–C) MTT assays for the glioma cells SWOZ2, SWOZ2-BCNU and U251 after TFP treatment. Cells were plated on 96-well plates and treated with different concentrations of TFP. The resulting number of viable cells was calculated by measuring the colour absorbance produced in each well. Data are the mean ± SD deviation of triplicate determinations. (D–F) Micrographic pictures of Fluo-3 fluorescence in SWOZ2, SWOZ2-BCNU and U251 cells treated with TFP at different time points. (G–I) The fluorescence intensity of Fluo-3-AM after TFP treatment in indicated cells at different time points. All values are presented as the mean ± SD of three experiments. *P < 0.05 compared with the control.
