Figure 3
PRP cold preconditioning, PRPr dilution, and plasma cryoprecipitate supplementation enhance angiogenesis mediated by PRP. PRP was incubated at 37 °C, 23 °C, or 4 °C for 30 min and then clotted by the addition of CaCl2 (22 mM) for 40 min. HMEC-1 (15 × 103) were incubated with PRPr pure (100%) or diluted with saline (25%). Saline containing 2% FBS was used as the control. Endothelial proliferation, migration, and tubule formation were determined in vitro without (A–C) or with plasma cryoprecipitate supplementation (D–F). (n = 5; **P < 0.01, ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. PRP 100%; &P < 0.05; &&P < 0.01 vs. 37 °C; ¥P < 0.05 vs. without plasma cryoprecipitate).
