Figure 4

Angiogenesis mediated by PRPr from diabetic patients is increased by optimisation. Platelet-poor-plasma (plasma) and PRP were obtained from blood samples obtained from healthy or diabetic patients. PRPr was prepared as non-optimised (PRPr: PRP pre-incubated at 37 °C; PRPr without dilution or supplements) or optimised PRP (O-PRPr: PRP pre-incubated at 4 °C; PRPr diluted and supplemented with plasma cryoprecipitate). Saline containing 2% FBS was used as the control. (A) Endothelial proliferation, (B) migration, and (C) tubule formation were determined in vitro (n = 8 per group; **P < 0.01, ***P < 0.001 vs. control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. PRPr; ^‡P < 0.05, ^‡‡&P < 0.01 vs. healthy same treatment).