Figure 6
PRP optimisation accelerates mouse skin regeneration. Four round full-thickness excisional wounds, 3 mm in diameter, were generated in the back skin of female BALB/c mice (8–10 weeks old). PRPr or O-PRPr obtained from other mice was injected subcutaneously in the periphery of the wounds. Saline was used as the control. Healing was analysed at 3, 7, 10, and 14 days post-injury. (A) Wounds were photographed, and the perimeter of the wound area was determined using the ImageJ software and expressed as a percentage of the area on day 0. (B) Skin biopsies were stained with Masson’s trichrome. Images were captured using an inverted microscope. (C) Epidermal thickness, (D) granulation tissue volume (dotted lines), (E) immature blood microvessels containing intraluminal erythrocytes, and (F) annex structures (hair follicles and sebaceous glands) were quantified using the ImageJ software. SG, sebaceous gland; HF, hair follicle; E, epidermis; D, dermis; F, fat layer; M, muscle layer; ST, subcutaneous tissue. (Magnification 100× and 200× , n = 4–15; *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. PRPr).
