Figure 7
Angiogenesis mediated by PRPr is inhibited by ASA and partially reversed by optimisation. PRP was incubated with ASA (0.1 or 0.5 mM) for 30 min, and then PRPr or O-PRPr was obtained and used to induce angiogenesis in vitro: (A) Endothelial proliferation, (B) migration, (C) tubule formation, and in vivo (D) blood vessel ramification over the CAM. (E) Microscopic CAM images represent one independent experiment. (n = 7–25, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. PRPr; ‡P < 0.05, ‡‡P < 0.01, ‡‡‡P < 0.001 vs. the same treatment without ASA).
