Figure 2
Box plots of absolute Cq values for each mRNA reference gene (A) and each npcRNA reference gene (B). Cells were cultured using RPMI 1640 medium containing 10% charcoal-stripped FBS for 24 hours, and then treated with different DHT concentrations. cDNA was prepared from these cells for the qPCR experiments. Expression of selected mRNA genes displayed as Cq across all prostate cell lines treated with various concentrations of DHT. The median is indicated by a line in each box, which in turn represents the 25th and 75th percentile. Whiskers indicate the 10/90 percentile ranges, and circles represent potential outliers.
