Table 1 PCR efficiency for all primer pairs of reference genes and target genes. R2 indicates correlation coefficient.
Gene | R2 | Efficiency | Slope |
|---|---|---|---|
18 S rRNA | 0.998 | 0.906 | −3.569 |
ACTB | 0.997 | 1.065 | −3.176 |
ALAS1 | 0.994 | 0.989 | −3.346 |
GAPDH | 0.997 | 0.924 | −3.519 |
HPRT1 | 0.993 | 0.997 | −3.330 |
K-ALPHA-1 | 0.998 | 0.987 | −3.354 |
RPL13A | 0.998 | 1.085 | −3.134 |
SDHA | 0.999 | 1.025 | −3.263 |
TBP | 0.995 | 1.025 | −3.264 |
YWHAZ | 0.997 | 1.009 | −3.300 |
PCA3 | 0.993 | 0.979 | −3.374 |
miR-16 | 0.999 | 0.917 | −3.539 |
miR-130b | 0.996 | 1.018 | −3.280 |
miR-1225-3p | 0.992 | 1.06 | −3.186 |
miR-1228-3p | 0.997 | 1.017 | −3.283 |
RNU-6-2 | 0.998 | 0.982 | −3.367 |
RNU-43 | 0.998 | 1.028 | −3.257 |
miR-141 | 0.999 | 0.975 | −3.383 |
