Table 3 Stability ranking of reference genes using geomean of ranking values analysed by three algorithms when prostate cells are treated with different DHT concentrations.
|  | Group | Gene | All treatments | 0 nM | 1 nM | 10 nM | 100 nM | 1000 nM |
|---|---|---|---|---|---|---|---|---|
mRNAgene | AR+celllines | ACTB | 1 | 1 | 3 | 2 | 5 | 1 |
GAPDH | 2 | 4 | 6 | 3 | 4 | 5 | ||
YWHAZ | 3 | 2 | 1 | 4 | 3 | 3 | ||
SDHA | 4 | 5 | 2 | 1 | 2 | 4 | ||
TBP | 5 | 3 | 4 | 6 | 6 | 6 | ||
K-ALPHA-1 | 6 | 7 | 8 | 5 | 1 | 8 | ||
18 S rRNA | 7 | 6 | 5 | 9 | 9 | 9 | ||
ALAS1 | 8 | 8 | 7 | 7 | 7 | 2 | ||
RPL13A | 9 | 9 | 9 | 8 | 8 | 7 | ||
HPRT1 | 10 | 10 | 10 | 10 | 10 | 10 | ||
The other cell lines | ACTB | 1 | 1 | 5 | 1 | 2 | 1 | |
HPRT1 | 2 | 4 | 1 | 4 | 3 | 2 | ||
SDHA | 3 | 5 | 3 | 2 | 1 | 4 | ||
RPL13A | 4 | 6 | 6 | 3 | 5 | 5 | ||
TBP | 5 | 2 | 2 | 6 | 6 | 3 | ||
ALAS1 | 6 | 3 | 4 | 7 | 8 | 7 | ||
K-ALPHA-1 | 7 | 8 | 7 | 8 | 4 | 8 | ||
YWHAZ | 8 | 7 | 8 | 9 | 9 | 6 | ||
GAPDH | 9 | 9 | 9 | 5 | 10 | 10 | ||
18 S rRNA | 10 | 10 | 10 | 10 | 7 | 9 | ||
npcRNAgene | AR+celllines | miR-16 | 1 | 2 | 1 | 2 | 3 | 1 |
miR-1228-3p | 2 | 3 | 2 | 1 | 1 | 3 | ||
miR-1225-3p | 3 | 1 | 4 | 3 | 2 | 2 | ||
RNU43 | 4 | 5 | 5 | 5 | 4 | 6 | ||
miR-130b | 5 | 6 | 3 | 4 | 5 | 5 | ||
RNU6-2 | 6 | 4 | 6 | 6 | 6 | 4 | ||
The other cell lines | RNU6-2 | 1 | 1 | 2 | 2 | 1 | 2 | |
RNU43 | 2 | 2 | 1 | 1 | 3 | 1 | ||
miR-1228-3p | 3 | 5 | 4 | 3 | 4 | 6 | ||
miR-16 | 4 | 3 | 3 | 4 | 2 | 4 | ||
miR-1225-3p | 5 | 4 | 6 | 6 | 5 | 3 | ||
miR-130b | 6 | 6 | 5 | 5 | 6 | 5 |
