Figure 4

Deficient IL-36/LXR axis in macrophages allows Mtb growth. (A–C) LXR luciferase activity of scramble versus IL36R-, LXRA-, LXRB-, CH25H-, CYP27A1-deficient macrophages after 8 h cell stimulation with (A) 500 nM of GW3965, 25HC, or 27HC, (B) 25 ng/ml rIL-36γ or (C) after 15 h Mtb infection. (D and E) Bacterial growth assessed by [3 H]-uracil incorporation (D) and CFUs (E) in IL-36 signaling deficient dual knockdown macrophages. (A–C) Data from one representative experiment of two independent experiments are shown. Data are shown as mean ± SD of technical replicates. (D and E) Bacterial growth results are representative of one experiment of two independent experiments with at least five replicates each. Data are shown as median ± interquartile range.