Figure 5
From: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
IL-36 signaling modulates cholesterol metabolism in Mtb-infected macrophage. (A) Changes in total cholesterol, free cholesterol and cholesteryl ester in cell lysates from scramble and IL36R KD THP-1 macrophages upon Mtb infection. (B) Immunofluorescence staining with Filipin III in non-infected (N/I), 24 h and 48 h Mtb-infected scramble versus IL36R KD THP-1 macrophages (40× objective magnification). (C) mRNA expression of CD36, LDLR, SRA in Mtb-infected scramble versus IL36R KD cells. (D) Cholesterol efflux upon Mtb infection or LXR stimulation in scramble versus IL36R KD THP-1 macrophages with or without LXR inhibitors. (E) SREBP2 protein expression in Mtb-infected scramble and IL-36 deficient cells. (F) mRNA expression of several cholesterol synthesis genes in Mtb-infected macrophages at 30 h with or without 27HC, 25HC and betulin stimulation. (A,C,D,F) Data pooled from three independent experiments are shown. Data are shown as mean ± SD. (B and E) Data from one representative experiment of at least two independent experiments are shown. P values: ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
