Figure 6

Oxysterols and inhibition of SREBP2 exhibit antibacterial effects. Quantification of (A) [3 H]-uracil uptake and (B) colony forming units (CFUs) of Mtb-infected scramble versus IL36R KD macrophages with simultaneous incubation of vehicle, 25HC, 27HC or betulin. (C and D) Bacterial viability in scramble, SREBF1 KD and SREBF2 KD macrophages at 120 h Mtb infection assessed by (C) [3 H]-uracil uptake assay and (D) CFU counts (E) mRNA expression levels of APs 24 h post Mtb infection of scramble and IL36R KD THP-1 macrophages, in the presence or absence of 25HC, 27HC or betulin. (A and B) Data from one representative experiment of three independent experiments are shown. (C and D) Data from one representative experiment of two independent experiments are shown, with at least five replicates each are shown. (E) Data pooled from three independent experiments are shown and presented as mean ± SD. P values: ns p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.