Figure 2

Modulation of FAO flux in aRCM. All aRCM were cultured in laminin-coated plates for 48 h; (A) FAO flux in untreated (control) adult rat cardiomyocytes (aRCM), or stimulated for 30 min with 5 μM oligomycin (Oli), or 3 μM rotenone (Rot), or 48 h with 10 μM of WY-14,643 (WY). Results are presented as mean ± SEM; ^$p < 0.05; ^$$p < 0.01; ^$$$p < 0.001 vs control (n = 17, 6, 2, 3). (B) Different stages of ¹⁴C-palmitate oxidation (adapted from¹⁷); (C) FAO flux in untreated fresh aRCM isolated from lean or zucker fatty (ZF) rats, n = 3 per group, ^$p < 0.05 vs lean; (D) FAO flux in 48 hours cultured aRCM from lean or ZF rats untreated or stimulated for 30 min with 5 μM oligomycin (Oli), n = 3 per group, ^$$p < 0.01 oligomycin vs untreated. FAO fluxes are calculated from the amount of radiolabeled palmitate that is converted into ¹⁴CO₂.