Figure 5

Peptide perturbations of model membranes are correlated to median percent helicity at 2 mol% peptide in DMPC and 2:1 DMPC:DMPG LUVs. Molar ellipticity of peptides at 222 nm was used to calculate peptide percent helicity in buffer and after the addition of LUVs at the specified molar ratio according to the method of Chen et al.¹⁷. A table was compiled (Supplementary material 03) and the median helicity of peptides after the addition of (a) DMPC and (b) 2:1 DMPC:DMPG LUVs was calculated as a function of cluster identity. (c) The relative position of peptides along the first principal component in PCA2 is linearly correlated to their percent helicity at 2 mol% in DMPC LUVs. The Pearson correlation between the relative position of peptides at Prin1 and their percent helicity when titrated with DMPC LUVs was r² = 0.67, ANOVA p < 0.0001). Regression equation: Helicity (%) = 31 (±2)-9 (±1) × Prin1. Peptide clusters are color coded: Cluster 1, in red, cluster 2 in blue, cluster 3 in green, cluster 4 in green and cluster 5 in gold.