Figure 2
From: High Density Lipoproteins Inhibit Oxidative Stress-Induced Prostate Cancer Cell Proliferation
AR activation is not involved in HDL antioxidant effect. LNCaP were treated overnight (o/n) with the androgen receptor antagonist bicalutamide (100 nM) before stimulation with 0.5 mM H2O2 for 1 h. HDL at 0.5 mg/ml were given overnight or as co-incubation with H2O2 (co-inc) for 1 h. ROS production was evaluated by fluorescence. Data are expressed as relative fluorescent units (RFU) normalized by the protein concentration of total cell lysate, mean ± SD, n = 6. #P < 0.05 vs H2O2-stimulated cells.
