Figure 4
From: High Density Lipoproteins Inhibit Oxidative Stress-Induced Prostate Cancer Cell Proliferation
SR-BI and ABCG1 are not involved in HDL antioxidant effect. Panel A, SR-BI and ABCG1 protein levels in PC-3 cells treated with specific or control (scrambled) siRNA. Image of SR-BI blot has been manipulated to eliminate empty lanes between control and SCARB1 siRNA samples and to show 3 bands/group as the original non manipulated blot for ABCG1. Images come from the same blots and same exposures. Original images of SR-BI are reported in Fig. S5. Panel B, ROS levels in H2O2-stimulated PC-3 cells pre-treated with specific or control (scrambled) siRNA. The effect of the co-incubation of 0.5 mM H2O2 with 0.5 mg/ml HDL for 1 h was tested. ROS production was evaluated by fluorescence. Data are expressed as relative fluorescent units (RFU) normalized by the protein concentration of total cell lysate, mean ± SD, n = 3. *P < 0.05 vs control cells incubated with scrambled siRNA, #P < 0.05 vs H2O2-stimulated cells incubated with scrambled siRNA, $P < 0.05 vs control cells incubated with ABCG1 or SCARB1 siRNA, §P < 0.05 vs H2O2-stimulated cells incubated with ABCG1 or SCARB1 siRNA.
