Figure 8
From: Control of neural crest induction by MarvelD3-mediated attenuation of JNK signalling
MarvelD3 depletion activates JNK signalling. Analysis of JNK signalling by immunofluorescence using an antibody against phosphorylated form of c-Jun (p-c-Jun) in animal caps injected with chd mRNA + wnt8 mRNA + control morpholino (A,C) or chd mRNA + wnt8 mRNA + MD3AB morpholinos (B,D). Nuclei were stained with Hoechst; red arrowheads: examples of nuclear co-localization between Hoechst and p-c-Jun staining; scale bar, 50 μm. (E) Quantification of the number of cells with a positive nuclear p-c-Jun staining / total number of cells. Mann-Whitney test p values and the total of cells counted (number in brackets) are indicated on the graph; the values are normalized to control morpholino + chd mRNA + wnt8 mRNA; black bar, chd mRNA + wnt8 mRNA + control morpholino; red bar, chd mRNA + wnt8 mRNA + MD3AB morpholinos.
