Figure 7
Gene expression validation in multiple donors. (A). Correlation plot of the normalized RNA-counts in the air controls from MucilAir™ donor MD059401 used in this study and MucilAir™ donor MD058501 used in a previous study13 and donor MD046001 from an unpublished study (raw sequence files provided). (B). Correlation plot of the Log2(FC) of the expressed genes found in common in the 3 MucilAir™ at 24 hrs post-exposure to cigarette smoke. Cells from MD58501 and MD059401 were exposed to smoke from 3R4F for an hour, cells from MD046001 were exposed to 1R6F cigarette smoke for an hour. The blue label indicates DEG (differentially expressed genes pFDR < 0.05) significant in the 3 donors, green labels have been used for DEGs significant in two donors, orange/pink labels have been used for DEGs significant in one donor, and the grey label have been used for gene not differentially expressed. Volcano plots for the qPCR validations of sets of 20 genes selected from the RNA-seq data performed with three donors showing 3R4F vs air (C) THS vs air (D) and THP1.0 vs air (E). The doted blue lines show the −log10(p-value = 0.05) and log2(FC = ±1.5) thresholds. Genes that were significant at these thresholds are annotated on the plots and colored in red for up-regulation and green for down-regulation.
