Figure 5

Staphylococcal LTA inhibits LPS-induced B cell proliferation in vivo. C57BL/6 mice (n = 3 per group) were i.p. injected with LPS (50 μg/mouse) and/or Sa.LTA (300 μg/mouse). After 48 h, spleens were photographed (a) and splenic leukocytes (b) and B220⁺ cells (c) were quantified using flow cytometric analysis. (d) TLR2-deficient C57BL/6 mice (n = 3 per group) were i.p. injected with LPS (50 μg/mouse) and/or Sa.LTA (300 μg/ml). After 48 h, spleens were removed and distribution of B220⁺ cells were analyzed using flow cytometric analysis. Data are expressed as the mean value ± standard deviation of results from three mice. (e) Mice (n = 3 per group) were i.p. injected with Sa.LTA (100 μg/mouse). After 24 h, spleens were removed and single cell suspensions were prepared as described in the Materials and Methods. Splenocytes were CFSE-labeled and stimulated with LPS (0.1 μg/ml) for 72 h. After stimulation, cells were stained with PerCP-conjugated rat anti-mouse CD45R/B220 antibody and B cell proliferation was determined by flow cytometry. The number indicates the percentage of cells in the designated areas. N.S. denotes not significant.