Figure 5
From: Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail
Identification of the RRM domain interacting with Lt2. (a,b) 1H-15N HSQC spectra of 15N-labelled Lt2 (100 μM) in the presence (red) and absence (black) of RRM1/2-poly(A) (200 μM) (a), and of RRM3/4-poly(A) (200 μM) (b). The peak labels in the parentheses in panel (a) are for the minor peaks of the split signals. (c,d) PRE results of 15N-labelled Lt2 in the presence of the A24-bound, spin-labelled RRM1/2/3/4. The intensity ratios of the 15N-Lt2 NMR signals are plotted in the presence of E29C‐MTSL (c) and D117C-MTSL (d). The intensity ratios were calculated by dividing the signal intensity of the radical (paramagnetic) form MTSL by the signal intensity of the reduced (diamagnetic) form of MTSL. *Indicates Pro or residues without assignments.
